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Job Field: Office Jobs
Location: Menlo Park, CA
Salary: $Not stated

JOB SUMMARY:

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<tr><td valign="top" width="450"><b>Job code 1213. Kinetic characterization of RNA-dependent polymerases and of other enzymes for SMRT RNA sequencing. Evaluate and elucidate detailed behavior of enzymes, both under bulk solution and SMRT sequencing conditions which includes evaluation of the direct, real time transcriptome sequencing performance. Data analysis of real-time single-molecule data.  Develop mutant enzymes for new RNA sequencing technologies.  Use state-of-the-art protein engineering methods, create polymerase diversity, and develop, implement and execute selection assays and processes for hit discovery and hit and lead optimization.  Analyze and interpret data from new and established assays systems, using robotics for assay development and application to high-throughput assay screening.  Apply genomic and proteomic data analysis capabilities to enable high throughput protein engineering. Work with enzymologists, molecular biologists, structural biologists, engineers and computer scientists to carry out responsibilities.
REQUIRED At least a graduate degree -Ph.D.- in Chemistry, Biochemistry or Molecular Biology. At least 2 years of postdoctoral experience. Skills  Develop assembly protocols for long RNA molecules for single-molecule studies of RNA and RNA-protein interactions. Single-molecule experiments involving RNA and proteins using a variety of single-molecule techniques, including data analysis. Biochemical studies of nucleic acid assembly and polymerase enzymology. Nucleic acid condensation.  Nucleic acid/protein isolation and purification. Use of  transmission electron microscopy for analysis of particle size and morphology. Dynamic light scattering. Atomic force microscopy. management of an RNA laboratory and funding of RNA-related projects.

KEY REQUIREMENTS:

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2 years Doctorate degree